Reference ranges for 1,25 (OH) 2 D may be reported as either pg/mL or pmol/L. The molecular weight of 1,25 (OH) 2 D is approximately 416.7, yielding the following conversion factors: 1 The consensus is that levels of 25(OH)D below 25nmol/L indicate 'deficiency'. Some have argued that this level is conservative. Raising the definition of "deficiency" or "sufficiency" to higher levels is inappropriate until results from randomised trials can show that maintaining such levels has clear health benefits and no health risks. What to expect. The 25-hydroxy vitamin D test involves providing a blood sample. To take a blood sample, a healthcare provider will first clean the skin on the person's arm. Then they will draw Vitamin D deficiency (serum 25-hydroxyvitamin D [25 (OH)D] < 50 nmol/L or 20 ng/ml) is associated with unfavorable skeletal outcomes, including fractures and bone loss. A 25 Vitamin D tests, which include immunoassays and liquid chromatography-mass spectrometry LC/MS/MS, measure the level of 25(OH)D, the main form of circulating vitamin D. Optimal levels Vitamin D levels of 30 to 100 ng/mL are considered optimal. The major circulating form of vitamin D is 25-hydroxyvitamin D (25(OH)D); thus, the total serum 25(OH)D level is currently considered the best indicator of vitamin D supply 5uRarm.

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